《植物生理学报》 2012, 48(3): 241-246

干旱胁迫下茶树基因表达的AFLP分析

孙云南, 陈林波, 夏丽飞, 李友勇, 田易萍, 梁名志*, 黄玫

云南省农业科学院茶叶研究所, 云南勐海666201

通信作者：梁名志；E-mail: liangmingzhi@126.com；Tel: 0691-5127079

摘　要：

利用cDNA-AFLP技术分析了茶树在干旱胁迫诱导下的基因表达差异, 通过256对引物组合共获得27个差异表达片段(TDF), 通过BLAST比对分析, 按其功能分为转录因子、基础代谢相关蛋白、抗逆蛋白、信号转导蛋白, 此外还有一些假设蛋白、未知蛋白和没有比对的基因片段。同时利用RT-PCR对片段GH2和GH15进行验证, 表明GH2和GH15片段均受到干旱胁迫诱导表达。这些研究结果显示茶树在干旱胁迫下的逆境反应非常复杂, 涉及多种代谢过程中的众多基因。

关键词：cDNA-AFLP; 茶树; 干旱胁迫; RT-PCR

收稿：2011-10-21   修定：2012-02-02

资助：安徽农业大学茶叶生物化学与生物重点实验室开放基金(ltbb201102041)和云南省农业科学院茶叶研究所项目(SL20100819-01)。

AFLP Analysis of Genes Expression in Tea (Camellia sinensis var. assamica) under Drought Stress

SUN Yun-Nan, CHEN Lin-Bo, XIA Li-Fei, LI You-Yong, TIAN Yi-Ping, LIANG Ming-Zhi*, HUANG Mei

Tea Research Institute of Yunnan Academy of Agricultural Science, Menghai, Yunnan 666201, China

Corresponding author: LIANG Ming-Zhi; E-mail: liangmingzhi@126.com; Tel: 0691-5127079

Abstract:

Differences of gene expression of tea plant under drought stress were studied by cDNA-AFLP. The results showed that 27 transcript-derived fragments (TDFs) were obtained. According to blasting results, these TDFs included transcription factors, primary metabolism proteins, proteins related to stress and signal transduction proteins. In addition, there were some genes for predicted protein and unknown protein and no significant homology found. The RT-PCR results showed that expression of GH2 and GH15 were both induced by drought stress. These results showed that response of tea plant to drought stress were very complex, involving many genes of several metabolism.

Key words: cDNA-AFLP; tea plant; drought stress; RT-PCR